PCIRN evaluation of pandemic H1N1 (2009) influenza vaccine in adults with human immunodeficiency virus infection
This study evaluated the safety and immune response of the pandemic H1N1 (2009) influenza vaccine in HIV-positive adults. Researchers assessed and compared which dosing level was best able to produce an optimal immune response to the vaccine. Researchers were looking to see if a booster dose of vaccine would increase the protection obtained by the vaccination.
A phase III, randomized trial was conducted at four Canadian sites. Two dosing strategies (standard dose vs standard dose plus booster on day 21) were assessed in HIV-positive participants aged 20 to 59 years during the H1N1 (2009) pandemic. A single antigen, inactivated split adjuvanted (AS03(A)) influenza vaccine (Arepanrix) was used. A test to determine the amount of the antigen in the blood serum was done at days 21 and 42 and at month 6.
HIV-positive individuals aged 20 to 59 years at four Canadian sites (Ottawa, Toronto, Montreal, Halifax).
One hundred and fifty participants received at least one injection. Within the two study arms both groups had similar baseline parameters: 83 per cent male, 85 per cent on HAART, median CD4 was equal to 519 cells/mm(3), 84 per cent with HIV RNA less than 50 copies/mL. At day 21, Seroprotection, the protection achieved by the vaccination was achieved in 80 per cent (95 per cent CI, 70-89) of participants. Seroconversion, the development of specific antibodies in the blood serum occurred in 74 per cent of participants (63-85). Seroprotection and seroconversion were further improved in those randomized to booster dosing: at day 42, 94 per cent (85-98) versus 73 per cent (60-83) (P < .01) and 86 per cent (75-93) versus 66 per cent (5-77) (P = .01). Seroprotection was retained in 40 per cent (28-54) of recipients at month six with trends toward greater retention of immunity in booster recipients. A smaller sub-study (64 participants) found that two doses of the vaccine is better at producing long-term (6 month) protection.
High-level immune response was achieved with a single dose of the vaccine. Immune response was further improved with booster dosing. Use of this vaccine and booster represent an important approach to increasing immune response in a population that has a diminished degree of responsiveness to vaccines. A two-dose immunization strategy may provide superior long-term protection for people living with HIV.
Dr. Curtis Cooper
Cooper C, Klein M, Walmsley S, Haase D, MacKinnon-Cameron D, Marty K, Li Y, Smith B, Halperin S, Law B, Scheifele D, PHAC CIHR Influenza Research Network. High-level immunogenicity is achieved with adjuvanted pandemic H1N1 vaccine and improved with booster dosing in a randomized trial of HIV-infected adults. HIV Clin Trials. 2012 Jan 13(1):23-32.
Thorne A, Chowdhury F, Singer J, Keynan Y, Fowke KR, Cooper C, CIHR Canadian HIV Trials Network (CTN) Influenza Vaccine Research Group. Comparison of hemagglutination inhibition and microbead array assays for the measurement of influenza antibody levels in HIV-infected adults. Vaccine. 2016;34(31):3584-3591.
Yam KK, Gipson E, Klein MB, Walmsley SL, Haase D, Halperin S, Scheifele D, Ward BJ, Cooper C. High Level Antibody Avidity is Achieved in HIV-Seropositive Recipients of an Inactivated Split Adjuvanted (AS03A) Influenza Vaccine. J Clin Immunol. 2014;34(6):655-662.
Scheifele DW, Dionne M, Ward BJ, Cooper C, Vanderkooi OG, Li Y, Halperin SA, Network PCIR. Safety and immunogenicity of 2010-2011 A/H1N1pdm09-containing trivalent inactivated influenza vaccine in adults previously given AS03-adjuvanted H1N1 2009 pandemic vaccine. Hum Vaccin Immunother. 2013;9(1):136-143.